Electrochemical system and method thereof

摘要

An embodiment of the invention provides an ultrasensitive and selective system and method for detecting reactants of the chemical/biochemical reaction catalyzed by an oxidoreductase, such as glucose and ethanol, at a concentration level down to zepto molar (10−21 M). In embodiments, the invention provides an ampyometric immuno-sensing system comprising a working electrode, an oxidoreductase, and an external voltage generator, wherein the oxidoreductase is immobilized on the working electrode; and the voltage generator generates a voltage to induce an electric field that permeates at least a portion of the interface between the oxidoreductase and the working electrode. The ultrasensitivity of the system and method is believed to be caused by the electrical field, which enhances the quantum mechanical tunneling effect in the interface, and therefore facilitates the interfacial electron transfer between the oxidoreductase and the working electrode.

主权项

1. An amperometric immuno-sensing system embodied in the form of an electrochemical cell system, the system comprising a working electrode, a reference electrode, and a counter electrode connected as a three-electrode cell, an enzyme-labeled antigen or an enzyme-labeled antibody, and an additional electrode, wherein the additional electrode is operatively connected to an external voltage source, wherein the enzyme-labeled antigen or the enzyme-labeled antibody is immobilized on the working electrode; and wherein the additional electrode is configured to apply an external voltage from the external voltage source between the additional electrode and the working electrode to induce an electric field, at least a portion of an interface between the enzyme-labeled antigen or the enzyme-labeled antibody and the working electrode being permeated by the electric field.