| 摘要 |
Methods of using polymerase chain reactions to enrich a plurality of target sequences in a sample containing reference sequences and target sequences having high homology and amplifiable by the same primer pairs are provided herein. In particular the methods provide a robust means to improve the fold enrichment of the target sequences and minimize reaction-to-reaction, well-to-well and run-to-run variations in the enrichment methods, e.g., in multiplex reactions. |
