METHODS AND COMPOSITIONS FOR INCREASING A T-EFFECTOR CELL TO REGULATORY T CELL RATIO

摘要

The present invention is directed to methods for increasing T-cell effector cell to regulatory T cell ratio. The invention is further directed to methods of treating, protecting against, and inducing an immune response against a tumor, comprising the step of administering to a subject a recombinant Listeria strain, comprising a fusion peptide that comprises an LLO fragment and tumor-associated antigen.

主权项

1. A method of eliciting an anti-tumor T cell response in a subject having a tumor or cancer, comprising the step of administering to said subject a recombinant Listeria strain comprising a recombinant nucleic acid, said nucleic acid molecule comprising a first open reading frame encoding a recombinant polypeptide and a second open reading frame second open reading frame encoding a metabolic, wherein said recombinant polypeptide comprises a truncated LLO protein fused to a heterologous antigen or fragment thereof, wherein said Listeria comprises a mutation in the endogenous alanine racemase gene (dal), D-amino acid transferase gene (dat), and actA genes, and wherein said T-cell response comprises increasing a ratio of T effector cells to regulatory T cells (Tregs), thereby eliciting an anti-tumor T cell response in said subject.