发明名称 |
METHODS FOR PREPARING A NEXT GENERATION SEQUENCING (NGS) LIBRARY FROM A RIBONUCLEIC ACID (RNA) SAMPLE AND COMPOSITIONS FOR PRACTICING THE SAME |
摘要 |
Methods of preparing a next generation sequencing (NGS) library from a ribonucleic acid (RNA) sample are provided. Aspects of the methods include combining the RNA sample with a first strand cDNA primer and a template switch oligonucleotide under first strand cDNA synthesis conditions, where one of the first strand cDNA primer and the template switch oligonucleotide includes a first post-tagmentation amplification primer binding domain. The resultant product is subjected to amplification conditions sufficient to produce a double stranded cDNA, which is then tagmented with a transposome that includes a second post-tagmentation amplification primer binding domain. The tagmented sample is then subjected to amplificationconditions using first and second post-tagmentation amplification primers that include sequencing platform adapter constructs to produce a NGS library. Aspects of the invention further include compositions produced by the methods and kits that find use in practicing the methods. |
申请公布号 |
WO2017048993(A1) |
申请公布日期 |
2017.03.23 |
申请号 |
WO2016US51989 |
申请日期 |
2016.09.15 |
申请人 |
TAKARA BIO USA, INC. |
发明人 |
CHANG, Cynthia;BOSTICK, Magnolia |
分类号 |
G06F19/10;C40B40/06;C40B40/08;G06F19/20;G06F19/22 |
主分类号 |
G06F19/10 |
代理机构 |
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地址 |
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