CELL-HOLDING SUBSTRATE HOLDER FOR PREPARING OBSERVATION SPECIMEN, KIT INCLUDING SAME, AND OBSERVATION SPECIMEN PREPARATION METHOD

摘要

A cell-holding substrate holder for preparing an observation specimen is provided with which the cell-holding substrate can be easily attached and detached. Also provided are: a cell-holding substrate kit; and an observation specimen preparation method with which cell organelles can be satisfactorily observed using an optical microscope, and the three problems of cell detachment, drying, and solidity loss can be simultaneously solved. The cell-holding substrate holder includes: (1) a support plate 1 provided with a cell-holding-substrate arrangement portion 12 provided with window portions through which water can pass; and (2) removable sandwiching plates 2, 3 which are provided with window portions through which water can pass, and are capable of working in conjunction with the support plate 1 to sandwich and fix the cell-holding substrate 9 in the cell-holding-substrate arrangement portion 12. The cell-holding substrate kit 10 includes the cell-holding substrate holder and the cell-holding substrate 9. In the observation specimen preparation method, cells are captured in an inorganic fibre aggregate, wet-fixed as they lie, dyed, and encapsulated using an encapsulant having the same refractive index as the inorganic fibres.