METHOD FOR DETECTING ESCHERICHIA COLI AND CARRIER FOR DETECTING ESCHERICHIA COLI

摘要

A method for simultaneously detecting enterohemorrhagic Escherichia coli and Escherichia coli (including enterohemorrhagic Escherichia coli) includes extracting genomic DNA from a sample; performing multiplex PCR for amplifying DNA fragments comprising a region of a uridine monophosphate kinase gene (pyrH) of Escherichia coli, DNA fragments comprising a region of a verotoxin 1 gene (vtx1) of Escherichia coli, and DNA fragments comprising a region of a verotoxin 2 gene (vtx2) of Escherichia coli, wherein the genomic DNA is used as a template; and simultaneously detecting presence or absence of each amplified product, whereby presence or absence of enterohemorrhagic Escherichia coli and Escherichia coli in the sample is simultaneously detected.

主权项

1. A method for simultaneously detecting enterohemorrhagic Escherichia coli and Escherichia coli (including enterohemorrhagic Escherichia coli), the method comprising:
extracting genomic DNA from a sample; performing multiplex PCR for amplifying DNA fragments comprising a region of a uridine monophosphate kinase gene (pyrH) of Escherichia coli, DNA fragments comprising a region of a verotoxin 1 gene (vtx1) of Escherichia coli, and DNA fragments comprising a region of a verotoxin 2 gene (vtx2) of Escherichia coli, wherein the genomic DNA is used as a template; and simultaneously detecting presence or absence of each amplified product, whereby presence or absence of enterohemorrhagic Escherichia coli and Escherichia coli in the sample is simultaneously detected.