| 摘要 |
The present invention relates to a method for producing ±-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of ±-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the ±-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing ±-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of ±-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of ±-glycated dipeptide is provided, which enables to determine the amount of ±-glycated dipeptide in a highly precise manner within a short time period. |
