Methods and systems for obtaining a single molecule consensus sequence

摘要

Computer systems, computer readable media, and computer methods for obtaining, calling, and assembling nucleic acid sequences are presented. In some aspects the invention includes the sequencing of template constructs that comprise double stranded portions in partially contiguous constructs, to provide for single molecule consensus sequence determination through one or both of sequencing sense and antisense strands in the same molecule.

主权项

1. A method for determining a single molecule consensus sequence of a target nucleic acid, the method comprising:
performing a single molecule nucleic acid sequencing of a strand of a single target nucleic acid molecule, thereby obtaining data,
wherein the strand of the single target nucleic acid molecule comprises a first region comprising a first nucleic acid sequence connected through a linking nucleic acid segment to a second region, the second region comprising a second nucleic acid sequence, the first nucleic acid sequence being the complement of the second nucleic acid sequence, andwherein the data comprises sequence data from the first region, the linking nucleic acid segment, and the second region; and assembling the single molecule consensus sequence of the target nucleic acid using the data.