Method for producing segmental aneuploidy (SAN) strains of <i>Trichoderma reesei </i>via sexual crossing and SAN strains produced therefrom

摘要

The present invention relates to a technology to provide segmental aneuploidy progeny strains of Trichoderma reesei. In particular, the present invention relates to a method to produce segmental aneuploidy progeny strains of Trichoderma reesei via sexual crossing of two parent haploid strains with chromosome heterozygosity (e.g. one having scaffold M and scaffold 33, the other having scaffold F and scaffold X), preferably at least one of which includes a non-homologous end joining (NHEJ) gene. The present invention also relates to stable, segmental aneuploidy progeny strains of richoderma reesei thus produced which particularly exhibit enhanced gene expression or activities of carbohydrate-active enzymes (CAZymes) and more particularly prevent returning to euploidy for an extended period of time.

主权项

1. A method for preparing a segmental aneuploid strain of Trichoderma reesei comprising steps of:
(a) identifying and choosing a first strain being Trichoderma reesei, which is mating competent and carries scaffold 33 and scaffold M in its genome; (b) identifying and choosing a second strain being Trichoderma reesei, which is mating competent, capable of mating with the first strain of step (a), and carries scaffold F and scaffold X in its genome; (c) sexually crossing the first strain of step (a) with the second strain of step (b); and (d) identifying and selecting a segmental aneuploid (SAN) progeny from step (c) that has duplication of the D segment in its genome.