System and method for autofocusing of an imaging system

摘要

Methods and systems for autofocusing of an imaging system are presented. Provided is an imaging system and an optical interferometry system for generating one or more images corresponding to a target region in a subject. The method provides calibration information that identifies a focal position of the optical interferometry system corresponding to a determined focal position of the imaging system. A subsequent focal position of the imaging system is determined for generating a desired image corresponding to at least one of another target region in the subject and another position of the target region relative to the imaging system based on the calibration information.

主权项

1. An autofocusing method, comprising:
providing an imaging system and an optical interferometry system for generating one or more images corresponding to a target region in a subject; determining calibration information that identifies a focal position of the optical interferometry system corresponding to a determined focal position of the imaging system at which the imaging system generates a desired image of the target region at an initial position, wherein determining calibration information comprises:
aligning the optical interferometry system with an objective element of the imaging system;determining an initial focal position of the imaging system at which the imaging system generates a desired image of the target region in the subject positioned at an initial position;determining location of the initial position of the target region using at least one image generated by the optical interferometry system;moving the target region out of focus of the optical interferometry system to reposition the target region at a subsequent position relative to the imaging system;refocusing the optical interferometry system on the repositioned target region and determine location of the subsequent position of the target region;computing a difference between the initial position and the subsequent position of the target region;moving the objective element by the computed difference to focus on the repositioned target region;determining a subsequent focal position of the objective element to focus on the repositioned target region at which the imaging system generates a desired image of the repositioned target region;measuring a difference between the subsequent focal position of the objective element and the computed difference to identify a calibration error between the optical interferometry system and the imaging system; andascertaining a distance by which the objective element is to be moved to generate the desired image of the repositioned target region based on the computed difference and the calibration error; determining a subsequent focal position of the imaging system for generating a desired image corresponding to at least one of another target region in the subject and another position of the target region relative to the imaging system based on the calibration information; and autofocusing the imaging system using the subsequent focal position to generate the desired image corresponding to at least one of the another target region in the subject and another position of the target region relative to the imaging system.