Polymerase compositions and uses

摘要

A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.

主权项

1. A composition having thermophilic nucleic acid polymerase activity, the composition comprising:
a) an active thermophilic nucleic acid polymerase selected from family A or family B present in the composition in a first amount, and b) a mutant thermophilic nucleic acid polymerase selected from family A or family B, present in the composition in a second amount greater than the first amount and having one to seven point mutations, wherein the mutant thermophilic nucleic acid polymerase has reduced nucleic acid synthesis activity and reduced substrate binding activity compared to the corresponding wild type thermophilic nucleic acid polymerase of (b) and wherein the mutant thermophilic nucleic acid polymerase has similar physical properties to the wild-type thermophilic nucleic acid polymerase including thermostability, enzyme surface hydrophobicity and other properties.