Temperature-responsive cell culture substrate and method for producing the same

摘要

A substrate having a pattern of two or more materials exhibiting different grafting efficiencies for a temperature-responsive polymer that varies its interaction with water in a temperature range of 0 to 80° C. by electron beam irradiation under the same conditions is grafted with the temperature-responsive polymer by simultaneously irradiating the surfaces of the materials with electron beams to obtain a temperature-responsive cell culture substrate. According to this method, a temperature-responsive cell culture substrate having a surface (1) that allows cells to adhere thereto and to grow thereon during cell culturing and that allows the adhering and grown cells to be detached therefrom by changing the culturing temperature and a surface (2) that does not allow the cells to adhere thereto at all can be obtained by a simple process.

主权项

1. A temperature-responsive cell culture substrate comprising: a substrate grafted with a temperature-responsive polymer that varies its interaction with water in a temperature range of 0 to 80° C. on a surface having two or more portions, wherein the two or more portions are comprised of different materials exhibiting different grafting efficiencies for the temperature-responsive polymer by electron beam irradiation under the same conditions, wherein the two or more portions comprise:
a portion (1) that allows cells to adhere thereto and to grow thereon during cell culturing and that allows the adhering and grown cells to be detached therefrom by changing the culturing temperature, and a portion (2) that does not allow the cells to adhere thereto at all; wherein the material of the portion (1) is any one selected from the group consisting of polystyrene, polycarbonate, and polymethyl methacrylate or a combination of two or more thereof; and the material of the portion (2) is any one selected from the group consisting of polymethylpentene, polyethylene terephthalate, polyethylene, polypropylene, and an ABS resin or a combination of two or more thereof.