Blood coagulation factor VII and VIIa derivatives, conjugates and complexes comprising the same, and use thereof

摘要

A blood coagulation factor VII derivative, a blood coagulation factor VIIa derivative, FacVII and FacVIIa conjugates are prepared by linking a polymer capable of extending the blood half-life to the derivative. FacVII and VIIa complexes each prepared by linking a carrier to the conjugate, genes encoding the FacVII and FacVIIa derivatives, expression vectors comprising the genes, transformants introduced with the expression vectors, a method for preparing the FacVII and FacVIIa derivatives using the transformants, a method for preparing the FacVIIa conjugate and complex, a FacVIIa complex prepared by the method, a pharmaceutical composition for the prevention or treatment of hemophilia comprising the derivative, conjugate, or complex as an active ingredient, and a pharmaceutical composition for blood coagulation comprising the derivative, conjugate, or complex as an active ingredient are described.

主权项

1. A derivative of blood coagulation factor VII (FacVII) or its active form FacVIIa, comprising the amino acid sequence of SEQ ID NO: 4 of the FacVII and a peptide linker, said peptide linker being linked to the FacVII or FacVIIa at the C-terminus of the FacVII or FacVIIa,
wherein the peptide linker consists of 6-149 consecutive amino acid residues starting from the amino acid residue at position 1 of SEQ ID NO: 30.