Antibody-immobilized carrier, method of producing antibody-immobilized carrier, and use of said antibody-immobilized carrier

摘要

The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.

主权项

1. A method of producing an antibody-immobilized carrier surface, comprising:
immobilizing one or more heavy-chain low-molecular-weight antibody fragments comprising a heavy-chain variable region (VH) fused to the N-terminus of a carrier binding peptide wherein the carrier binding peptide is selected from the group consisting of SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18 and SEQ ID NO: 19; and immobilizing one or more light-chain low-molecular-weight antibody fragments comprising a light-chain variable region (VL) fused to the N-terminus of a carrier binding peptide wherein the carrier binding peptide is selected from the group consisting of SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18 and SEQ ID NO: 19 sequentially onto a carrier surface, wherein the material of the carrier surface is selected from the group consisting of a polystyrene plastic resin, a polycarbonate plastic resin, a polymethyl methacrylate plastic resin, a plastic resin obtained through hydrophilizing a polystyrene plastic resin, a plastic resin obtained through hydrophilizing a polycarbonate plastic resin, and a plastic resin obtained through hydrophilizing a polymethyl methacrylate plastic resin, so that an antibody immobilized carrier surface is prepared.