| 发明名称 |
NOVEL COMPOSITION FOR ASTRINGIN PRODUCT |
| 摘要 |
Provided is a composition for producing astringin among metabolites of polydatin, wherein the astringin may be mass-produced by oxidizing the polydatin using a CYP102A1 chimera and mutants thereof as a catalyst, the CYP102A1 chimera being produced by fusing a reductase domain of a wild-type CYP102A1 which is a bacterial cytochrome P450 enzyme, with a heme domain of a CYP102A1 mutant. |
| 申请公布号 |
US2017073720(A1) |
申请公布日期 |
2017.03.16 |
| 申请号 |
US201615145679 |
申请日期 |
2016.05.03 |
| 申请人 |
INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY |
发明人 |
Yun Chul-Ho;Kang Hyung-Sik;Joung Young-Hee;Cha Gun Su |
| 分类号 |
C12P19/46;C12N9/02 |
主分类号 |
C12P19/46 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A composition for producing astringin comprising at least one enzyme of the group consisting of mutants of CYP102A1 chimera and the CYP102A1 chimera, and producing astringin by oxidizing polydatin,
wherein the CYP102A1 chimera is R47L/F81I/F87V/E143G/L188Q/E267V/A475V/E559D/T665A/P676L/A679E/E688A/A742G/K814E/R826S/R837H/E871N/1882V/E888G/P895S/S955N/M968V/Q982R/A1009D/H1022Y/Q1023E of a CYP102A1 mutant, the CYP102A1 mutant is R47L/F81I/F87V/E143G/L188Q/E267V of a wild-type CYP102A1, and the mutant of CYP102A1 chimera is at least one selected from the group consisting of F11L/R47L/F81I/F87V/Q10P/E143G/L188Q/R190Q/E267V, R47L/F81I/F87V/L103F/D136G/E143G/N159S/L188Q/E267V, and R47L/F81I/F87V/M112T/E143G/L188Q/E267V/M417T of the CYP102A1 chimera. |
| 地址 |
Gwangju KR |
