TRANSAMINASE POLYPEPTIDES

摘要

The present disclosure provides engineered transaminase enzymes having improved properties as compared to a naturally occurring wild-type transaminase enzyme. Also provided are polynucleotides encoding the engineered transaminase enzymes, host cells capable of expressing the engineered transaminase enzymes, and methods of using the engineered transaminase enzymes to synthesize a variety of chiral compounds.

主权项

1. An engineered polynucleotide encoding a transaminase polypeptide, wherein said transaminase polypeptide comprises a polypeptide sequence having transaminase activity, wherein the encoded transaminase polypeptide comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:2, and wherein the transaminase polypeptide comprises a substitution at position 45.