ARTICLE AND METHOD FOR DETECTING AEROBIC BACTERIA

摘要

A thin film culture device for detecting aerobic bacteria in a sample is provided. The culture device comprises a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet, a sample-receiving zone disposed between the substrate sheet and the cover sheet, a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to a portion of the sample-receiving zone; and a plurality of indicator agents disposed in at least one layer adhered to the substrate sheet or the cover sheet. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, an indicator agent for detecting a phosphatase enzyme activity, and a redox indicator. A method of using the culture device is also provided.

主权项

1. A device for culturing and detecting microorganisms, the device comprising:
a self-supporting substrate sheet having a first major surface and a second major surface; a cover sheet attached to the substrate sheet; a sample-receiving zone disposed between the substrate sheet and the cover sheet; a first layer comprising a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first major surface of the substrate sheet; and a plurality of indicator agents, the plurality of indicator agents comprising:
three enzyme activity indicator reagents for detecting distinct glycosidase enzyme activities;an enzyme activity indicator reagent for detecting an alkyl esterase enzyme activity;an enzyme activity indicator reagent for detecting a phosphatase enzyme activity;a redox indicator comprising a tetrazolium dye; wherein each of the plurality of enzyme activity indicator reagents comprises a detectable reporter group; wherein each of the plurality of indicator agents is disposed in at least one layer adhered to the substrate sheet or the cover sheet, wherein the at least one layer is in fluid communication with the sample-receiving zone when a predetermined volume of aqueous liquid is deposited in the sample-receiving zone.