| 摘要 |
The present invention pertains to a method of isolating RNA from a sample comprising RNA, and DNA, comprising:
a) adding an acidic denaturing composition comprising a chaotropic agent and phenol to the sample;
b) adding a water-insoluble organic solvent and separating the resulting phases thereby forming a multi-phase mixture comprising an aqueous phase, optionally an interphase and an organic phase, wherein the RNA is concentrated in said aqueous phase and DNA and proteins are concentrated in said organic phase and/or in said interphase; and
c) isolating said RNA from said aqueous phase,
wherein at least one cationic detergent is added before separating the phases. It was found that the addition of at least one cationic detergent considerably reduces the amount of DNA in the aqueous, RNA containing phase. Therefore, the present invention allows to easily isolate pure RNA which comprises considerably less DNA contaminations. |
