| 摘要 |
The present invention pertains to method for purifying at least a target nucleic acid from a sample, said method comprising at least the following steps
a) incubating the sample with at least one protein-degrading compound,
b) binding the target nucleic acid to a solid phase,
c) eluting the target nucleic acid from the solid phase,
d) incubating the eluted target nucleic acid with at least one protein-degrading compound,
e) binding the target nucleic acid again to a solid phase
f) optionally eluting the bound target nucleic acid from the solid phase.
It was surprisingly found that performing a second protein digestion step after the target nucleic acid was bound and eluted from a solid phase before the nucleic acids are rebound to a solid phase is very efficient in reducing remaining protein contaminations in the isolated nucleic acid. |
