RECOMBINANT ESCHERICHIA COLI GENERATED D-LACTIC ACID, AND USE THEREOF

摘要

Provided is recombinant Escherichia coli comprising a mutated ldhA gene. Disclosed are use of the recombinant Escherichia coli in the preparation of D-lactate, and method of preparing D-lactate by employing the recombinant Escherichia coli. In the method of preparing D-lactate, fermentation can be conducted at a high temperature (42-50 °C), and aqueous ammonia or sodium hydroxide can be used as a neutralizer during the fermentation.