发明名称 |
NOVEL VERTEBRATE CELLS AND METHODS FOR RECOMBINANTLY EXPRESSING A POLYPEPTIDE OF INTEREST |
摘要 |
The present application pertains inter alia to an isolated vertebrate cell suitable for recombinant expression of a polypeptide of interest, wherein the vertebrate cell is altered to impair the function of the endogenous protease matriptase and wherein said cell comprises at least one heterologous polynucleotide encoding a polypeptide of interest and wherein the polypeptide of interest is secreted by the cell. It was found that using respective vertebrate cells for producing a recombinant polypeptide of interest significantly reduces clipping of the polypeptide of interest that is secreted into the cell culture medium. Also provided are improved production and screening methods. |
申请公布号 |
EP3137595(A2) |
申请公布日期 |
2017.03.08 |
申请号 |
EP20150739327 |
申请日期 |
2015.04.29 |
申请人 |
Novartis AG |
发明人 |
LAUX, Holger;ROMAND, Sandrine;BODENDORF, Ursula |
分类号 |
C12N5/00;C12N5/071;C12N15/67;C12N15/85;C12N15/87 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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