Biosynthesis of human milk oligosaccharides in engineered bacteria

摘要

The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

主权项

1. An isolated E. coli bacterium comprising
(i) a deletion or functional inactivation of an endogenous β-galactosidase gene; (ii) an exogenous functional β-galactosidase gene comprising a detectable level of β-galactosidase activity that is reduced compared to that of a wild-type E. coli bacterium, wherein the level of β-galactosidase activity comprises between 0.05 and 200 units; (iii) an inactivating mutation in a colanic acid synthesis gene; and (iv) an exogenous lactose-accepting fucosyltransferase gene.