Chimeric primers with hairpin conformations and methods of using same

摘要

Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5′ of the target-specific primer sequence; a tag sequence 5′ of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.

主权项

1. A nucleic acid molecule comprising:
(a) a target-specific primer sequence; (b) an anti-tag sequence 5′ of the target-specific primer sequence; (c) a tag sequence 5′ of the anti-tag sequence; and (d) a blocker between the anti-tag sequence and the tag sequence.