发明名称 |
HIGH-YIELD PEPTIDE ANTIBIOTICS PRODUCING STRAIN, PREPARATION METHOD AND USE THEREOF |
摘要 |
High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture A; (b) mixing said mixture A with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization. |
申请公布号 |
CA2821791(C) |
申请公布日期 |
2017.03.07 |
申请号 |
CA20112821791 |
申请日期 |
2011.12.15 |
申请人 |
SHANGHAI TECHWELL BIOPHARMACEUTICAL CO., LTD. |
发明人 |
CHEN, YI;LIU, SHIDONG;ZHANG, ZHAOLI;WANG, CHUNXIA;KANG, JING;JI, XIAOMING |
分类号 |
C12N1/14;C12P21/04 |
主分类号 |
C12N1/14 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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