Methods of producing pancreatic hormones

摘要

Disclosed herein are methods of producing pancreatic hormone-expressing cells by first differentiating pluripotent cells in cell culture so as to produce endodermal cells, the endodermal cells being competent to further differentiate into hormone-expressing cells capable of secreting at least one pancreatic hormone in response to a physiological signal, and then, transplanting the cultured endodermal cells into an organism, such as an organism in need of an endocrine cell therapy.

主权项

1. A method for lowering blood glucose levels in a mammal, comprising the steps of:
(a) obtaining a population of human definitive endoderm cells; (b) differentiating the population of definitive endoderm cells into a population of pancreatic endoderm and endocrine precursor cells using a medium comprising glucose, wherein the population expresses NKX6.1 and PDX1; (c) transplanting the population of pancreatic endoderm and endocrine precursor cells into the mammal; and (d) maturing the transplanted cells of step (c) into insulin secreting cells.