Microfluidic system and method with focused energy apparatus

摘要

An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.

主权项

1. An apparatus which identifies objects, said apparatus comprising:
a microfluidic chip in which are disposed a plurality of channels, including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, said sheath fluids which orient said objects in said main fluid channel in a predetermined direction while still maintaining laminar flow in said main fluid channel; wherein said plurality of sheath fluid channels comprise: a first plurality of sheath fluid channels which intersect said main fluid channel at a first intersection, such that said sheath fluids compress said sample fluid mixture on at least two sides, such that said fluid mixture becomes a relatively smaller, narrower stream, bounded by said sheath fluids, while maintaining laminar flow in said main fluid channel; a second plurality of sheath fluid channels which intersect said main fluid channel at a second intersection downstream from said first intersection, such that said sheath fluids from said second plurality of sheath fluid channels compress said sample fluid mixture in one of said at least two sides, or in two sides opposite from said at least two sides, such that said sample fluid mixture is further compressed while still maintaining laminar flow in said main fluid channel; and a third sheath fluid channel disposed vertical to said main fluid channel at a third intersection, and disposed downstream from said second intersection, said sheath fluid from said third sheath fluid channel which further compresses said sample fluid mixture while still maintaining laminar flow in said main fluid channel; an interrogation apparatus which detects and interrogates said oriented objects in said main fluid channel; and a focused energy apparatus which performs an action on said objects.