DNA SEQUENCING USING MULTIPLE METAL LAYER STRUCTURE WITH DIFFERENT ORGANIC COATINGS FORMING DIFFERENT TRANSIENT BONDINGS TO DNA

摘要

A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.

主权项

1. A nanodevice, comprising:
a reservoir filled with a conductive fluid; a membrane separating the reservoir, the membrane comprising electrode layers separated by insulating layers; and a nanopore formed through the membrane, a certain electrode layer having a first type of organic coating and a pair of electrode layers having a second type of organic coating inside the nanopore; wherein the first type of organic coating on the certain electrode layer forms a motion control transient bond to a molecule in the nanopore for motion control of the molecule; wherein the second type of organic coating on the pair of electrode layers forms a first transient bond to a first base and a second transient bond to a second base of the molecule in the nanopore; wherein, when a voltage is applied to the pair of electrode layers a tunneling current is generated though the first base along the molecule to the second base in the nanopore, the tunneling current traveling via the first and the second transient bonds respectively to be measured as a current signature for distinguishing the first base and the second base; and wherein the motion control transient bond is stronger than the first and the second transient bonds.