发明名称 |
SYSTEM FOR GENERATING DROPLETS WITH PUSH-BACK TO REMOVE OIL |
摘要 |
System, including methods, apparatus, and kits, for forming and concentrating emulsions. An exemplary system may comprise a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, a droplet well, and a channel network interconnecting the wells. The system also may comprise an instrument configured to operatively receive the device and to create (i) a first pressure differential to produce an emulsion collected in the droplet well and (ii) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well. |
申请公布号 |
US2017056884(A1) |
申请公布日期 |
2017.03.02 |
申请号 |
US201615351331 |
申请日期 |
2016.11.14 |
申请人 |
Bio-Rad Laboratories, Inc. |
发明人 |
Hiddessen Amy L.;Masquelier Donald A.;Ness Kevin D.;Hindson Benjamin J.;Makarewicz, JR. Anthony J.;Chia Erin R. |
分类号 |
B01L3/00;B01F13/00;C12Q1/68;B01F3/08 |
主分类号 |
B01L3/00 |
代理机构 |
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代理人 |
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主权项 |
1. A system to form and concentrate an emulsion, comprising:
a device including a sample well configured to receive sample-containing fluid, a continuous-phase well configured to receive continuous-phase fluid, and a droplet well, the device also including a channel network having a first channel, a second channel, and third channel that meet one another in a droplet-generation region; and an instrument configured to operatively receive the device and to create
(a) a first pressure differential to drive sample-containing fluid from the sample well to the droplet-generation region via the first channel, continuous-phase fluid from the continuous-phase well to the droplet-generation region via the second channel, and sample-containing droplets from the droplet-generation region to the droplet well via the third channel, such that the droplet well collects an emulsion including sample-containing droplets disposed in continuous-phase fluid, and(b) a second pressure differential to decrease a volume fraction of continuous-phase fluid in the emulsion, after the emulsion has been collected in the droplet well, by selectively driving continuous-phase fluid, relative to sample-containing droplets, from the droplet well via the third channel. |
地址 |
Hercules CA US |