METHOD FOR DETECTING GENES SENSITIVE TO LOW-LEVEL IONIZING RADIATION, AND GENE DETECTED BY THE METHOD

摘要

A method for detecting genes sensitive to low-level ionizing radiation and genes detected by the method. More specifically, genes sensitive to low-level ionizing radiation, discovered in a carcinogenic entity and verified in a normal entity are detected by subjecting a cancerous AKR/J mouse and a normal ICR mouse to low-level radiation. Thymus is collected therefrom, and glycometabolism-related genes are classified via microarray processing of the thymus. The genes are amplified and the levels of gene expression are measured. Thus, a gene having a specific reaction to radiation can be accurately detected by preventing the interference of confounding variables.

主权项

1. A method for detecting a gene sensitive to an ionizing radiation, the method comprising the steps of:
irradiating a cancer-induced AKR/J mouse and an ICR mouse with a gamma radiation at a dose rate of a dose rate of 0.7 mGy/hr to a final dose of 1.7 Gy; extracting from the AKR/J mouse and the ICR mouse; subjecting the thymi to a microarray analysis; selecting an IRS1 gene as a glucose metabolism-related gene from the microarray analysis; and amplifying the IRS1 gene and measuring an expression level of the gene.