SYNTHETIC BRASSICA-DERIVED CHLOROPLAST TRANSIT PEPTIDES

摘要

This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.

主权项

1. An isolated nucleic acid molecule comprising:
a polynucleotide that encodes a synthetic chloroplast transit peptide (CTP) comprising: a contiguous amino acid sequence of a first CTP, wherein the first CTP is from a Brassica 3-enolpyruvylshikimate-5-phosphate synthetase (EPSPS); and a contiguous amino acid sequence of a second CTP, wherein the second CTP is from a different EPSPS.