主权项 |
1. An apparatus for detecting or quantifying a plurality of analytes of interest in a fluid sample suspected of containing the plurality of analytes of interest, the apparatus comprising:
a microfluidic or nanofluidic system comprising:
a sample inlet that, in use, receives the fluid sample;a mixing chamber that, in use, mixes the sample with one or more selective binding fluorescent labels;a detection chamber comprising a first volume and a second volume, the first volume comprising a first immobilized selective binding species and the second volume comprising a second immobilized selective binding species; anda sample outlet, wherein the sample inlet, the mixing chamber, the detection chamber, and the sample outlet are fluidly connected; and an optical system comprising:
a light source configured to illuminate the first volume and the second volume;an optical detector configured to receive and detect emitted radiation from the first volume and the second volume; anda light source attenuator positioned between the detection chamber and the optical detector. |