METHODS FOR DISCRIMINATING INTERSPECIFIC RELATIONSHIPS AND INTRASPECIFIC RELATIONSHIPS OF LACTIC ACID BACTERIA OF LACTOBACILLUS CASEI GROUP

摘要

Degenerate primers for amplifying fragments of the nucleotide sequences of mutL and dnaJ genes of a lactic acid bacterium of Lactobacillus casei group are provided. Methods and kits for discriminating interspecies and intraspecies of a lactic acid bacteria of Lb. casei group are also provided.

主权项

1. A degenerate primer pair comprising:
a first primer mixture comprising the nucleotide sequences shown as 5′-ATTGAYCARCTATCKGCMAC-3′ (SEQ ID NO: 1), wherein Y=C or T; R=A or G; K=G or T; and M=A or C, and a second primer mixture comprising the nucleotide sequences shown as 5′-GAGCCRTAACCGGCAATMA-3′ (SEQ ID NO: 2), wherein R=A or G; and M=A or C; or a first primer mixture comprising the nucleotide sequences shown as 5′-TCTGAAYTTVGTYTGRCTTTG-3′ (SEQ ID NO: 3), wherein Y=C or T; V=G, A or C; and R=A or G, and a second primer mixture comprising the nucleotide sequences shown as 5′-GTGGTGCVCARGCKAATC-3′ (SEQ ID NO: 4), wherein V=G, A or C; R=A or G; and K=G or T.