Plant Pattern Recognition Receptor And Chimeras Thereof For Use Against Bacterial Infections

摘要

The present invention relates to the finding that an until now orphan protein named receptor-like protein 1 (RLP1) in plants mediates an immune response to bacterial infections. Specifically, the invention relates to RLP1, now named receptor of enigmatic microbe-associated molecular pattern (MAMP) of Xanthomonas (REMAX), in Arabidopsis thaliana REMAX was found to recognise the presence of Xanthomonas and to initiate an immune signalling that eventually yields into a typical plant immune responses to bacterial infections. Furthermore, the invention relates to chimeric pattern recognition receptors (PRRs) composed of the extracellular domain of REMAX, which is the recognition site for sensing infection, and c-terminal portions of PRRs of other plant species. Also provided is a method to modulate the immune response of a plant to a bacterial infection by either increasing or decreasing the expression of REMAX or REMAX-like proteins, or the inventive chimeric PRRs in plants. Finally, the invention provides methods for the identification and purification of new microbe-associated molecular patterns in plants using REMAX or REMAX-like proteins and/or the novel chimeric PRRs of the present invention.

主权项

1. A chimeric pattern recognition receptor (PRR) for recognizing plant pathogen-associated molecular patterns, comprising at least an ectodomain derived from the protein shown in SEQ ID No. 1, or a protein that is at least 50% identical to the protein shown in SEQ ID No. 1, a juxtamembrane domain, a transmembrane domain and a cytoplasmatic domain, characterized in that the ectodomain and at least one of the other domains are derived from different PRRs.