| 摘要 |
A cell analysis method for analyzing, using a flow cytometer, cells obtained from a tissue specimen which is aldehyde-fixed and embedded in a water-insoluble embedding medium. The method comprises: (a) removing embedding medium from a sample obtained from the tissue specimen which is aldehyde-fixed and embedded in the water-insoluble embedding medium, thereby obtaining the sample comprising the tissue from which the embedding medium is removed; (b) heating the sample obtained from step (a) in the presence of a divalent carboxylic acid compound to obtain a heat-treated sample comprising the tissue; (c) contacting the heat-treated sample from step (b) with an enzyme having a cell dispersion activity to separate the sample into individual cells, thereby obtaining the sample comprising the individual cells dispersed in a solvent; (d) subjecting the separated cells from step (c) to flow cytometry to obtain optical information ; and (e) analyzing the cells based on the optical information obtained in the step (d). |
