发明名称 |
Method for preparation of site-specific protein conjugates |
摘要 |
The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB 1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer. |
申请公布号 |
EP2599502(B1) |
申请公布日期 |
2017.03.01 |
申请号 |
EP20120190040 |
申请日期 |
2004.04.08 |
申请人 |
ANTRIABIO, INC. |
发明人 |
Hinds, Kenneth;Lewis, Danny;Schmidt, Paul;Campbell, Kathleen M |
分类号 |
A61K47/60;A61K;A61K9/16;A61K31/00;A61K38/28;A61P1/18 |
主分类号 |
A61K47/60 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|