Joining and/or sealing tissues through photo-activated cross-linking of matrix proteins

摘要

A method of joining and/or sealing tissues in a surgical procedure or medical treatment, comprising the steps of: (1) applying a matrix protein, a photoactivatable metal-ligand complex and an electron acceptor to a tissue portion; (2) irradiating said tissue portion to photactivate the photoactivatable metal-ligand complex: thereby initiating a cross-linking reaction of the matrix protein to seal said tissue portion or join said tissue portion to an adjacent tissue portion.

主权项

1. A method of treating damaged tissue, the method comprising:
applying a closure, the closure comprising a substrate suitable for application to a wound to stem bleeding, wherein said substrate is selected from the group consisting of a collagen sheet, bandage, gauze, cloth, tampon, membrane or sponge and is impregnated or coated with a tissue adhesive, said tissue adhesive comprising a matrix protein selected from the group consisting of fibrinogen, fibrin, collagen, fibronectin, keratin and laminin, or admixtures thereof, a photoactivatable metal-ligand complex and an electron acceptor; and irradiating the closure, thereby causing a cross-linking reaction between one of more matrix proteins within or coating the closure and the surrounding patient tissue.