| 摘要 |
New strains of microalgae belonging to the Nitzschia genus, allow high-yield production of lipids, in particular of docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA) and/or carotenoids, in particular fucoxanthin, in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light. |
| 主权项 |
1. A method for increasing the production of lipids and carotenoids in a culture of microalgae of the genus Nitzschia, comprising the following step:
a) culturing, in mixotrophic mode, of one or more strains of microalga of the genus Nitzschia, over several generations in a culture medium comprising a carbon-containing substrate selected from the group consisting of lactate, lactose, saccharose, acetate, glycerol, glucose, cellulose derivatives and mixtures thereof, under conditions of alternation of illuminated phases and phases of darkness, wherein illumination is provided in the form of flashes of light, said flashes having a duration of between 5 seconds and 10 minutes, with an intensity—between 5 and 1,000 μmol·m−2·s−1, said flashes occurring between 2 and 3,600 times per hour, and wherein the phases of darkness occupy more than half of the time during which the microalgae are cultured; and b) recovering the cultured Nitzschia microalgae, the microalgae being charged with said lipids and carotenoids. |
