Kidney structures and methods of forming the same

摘要

Provided herein are isolated populations of kidney cells harvested from differentiated cells of the kidney, wherein cells have been expanded in vitro, and methods of use thereof. The cells may be provided in a three dimensional matrix for culturing in vitro and/or implanting in vivo. Methods of seeding cells onto the matrix are also provided.

主权项

1. A method of treating a kidney disease in a patient in need thereof, said method comprising:
providing a composition comprising an isolated population of differentiated mammalian kidney cells comprising peritubular interstitial cells, wherein said cells are harvested from differentiated cells of a mammalian kidney tissue, passaged in vitro from 2 to 9 times, and seeded onto a three dimensional matrix, wherein (i) the cells of said population produce erythropoietin (EPO) under normoxic conditions, and (ii) the differentiated cells of the mammalian kidney tissue or the population of cells have not been manipulated by the introduction of an exogenous gene that stimulates the production of EPO or by an exogenous chemical that stimulates the production of EPO; and administering said composition to said patient in an amount effective to treat said kidney disease by surgically implanting said composition subcutaneously or into the kidney of said patient, to thereby treat said kidney disease in said patient in need thereof.