Attenuated live bacteria with increased acid resistance and methods of use thereof

摘要

The present invention relates to inducing acid resistance in a bacterium and methods of increasing the acid resistance of an acid sensitive bacterium.

主权项

1. A recombinant attenuated derivative of a pathogenic enteric Salmonella bacterium comprising a nucleic acid encoding an antigen and at least one of the following:
a) a regulatable promoter operably linked to a nucleic acid encoding a Salmonella AdiA arginine decarboxylase and a nucleic acid encoding a Salmonella AdiC arginine agmatine antiporter; b) a regulatable promoter operably linked to a nucleic acid encoding an E. coli GadB and/or an E. coli GadA glutamate decarboxylase and a nucleic acid encoding an E. coli GadC glutamate/γ-aminobutyric acid antiporter; or c) a regulatable promoter operably linked to a nucleic acid encoding a Salmonella CadA lysine decarboxylase and a nucleic acid encoding a Salmonella CadB lysine/cadaverine anti porter.