Thermostable cellobiohydrolase and amino acid substituted variant thereof

摘要

A thermostable cellobiohydrolase including a cellobiohydrolase catalytic domain, the cellobiohydrolase catalytic domain including:;(A) a polypeptide including an amino acid sequence represented by SEQ ID NO: 1;;(B) a polypeptide including an amino acid sequence obtained by deletion, substitution or addition of at least one amino acid of the amino acid sequence represented by SEQ ID NO: 1, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5;;(C) a polypeptide including an amino acid sequence having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO: 1, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5;;(D) a polypeptide including an amino acid sequence represented by SEQ ID NO: 3;;(E) a polypeptide including an amino acid sequence obtained by deletion, substitution or addition of at least one amino acid (with a proviso that cysteine residues at positions 291 and 296 in the amino acid sequence are excluded) of the amino acid sequence represented by SEQ ID NO: 3, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5; or;(F) a polypeptide including an amino acid sequence (with a proviso that cysteine residues are present at positions 291 and 296) having at least 85% sequence identity with the amino acid sequence represented by SEQ ID NO: 3, and having at least a cellobiohydrolase activity under conditions of 75° C. and pH 5.5.

主权项

1. A thermostable cellobiohydrolase comprising:
(A) a polypeptide consisting of an amino acid sequence of SEQ ID NO: 1 or (B) a polypeptide consisting of an amino acid sequence of SEQ ID NO: 3; and at least one domain selected from the group consisting of a cellulose binding module, a linker domain, a signal peptide, and a tag.