Fusion tags and expression vector system for the expression of human parathyroid hormone (rhPTH)

摘要

The present invention provides for fusion tags of 20 to 40 amino acids having a sequence as given in FIG. 1, fusion tags sharing homology with these fusion tags, or hybrid fusion tags comprising sequences of FIG. 1. The invention further provides an expression vector system comprising a fusion tag of the invention, and a process of obtaining recombinant human parathyroid hormone by using the fusion tags and the expression vector system of the invention.

主权项

1. A recombinant fusion tag of 20-40 amino acid for expression and purification of recombinant human parathyroid hormone (rhPTH), said fusion tag comprising an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence set forth in SEQ ID NO.1; (b) the amino acid sequence set forth in SEQ ID NO.2; (c) the amino acid sequence set forth in SEQ ID NO.3; (d) the amino acid sequence set forth in SEQ ID NO.4; (e) the amino acid sequence set forth in SEQ ID NO.5; (f) the amino acid sequence set forth in SEQ ID NO.6; and (g) the amino acid sequence set forth in SEQ ID NO.7.