Chimeric poly peptides and the therapeutic use thereof against a Flaviviridae infection

摘要

The invention relates to building a chimeric polypeptide used for preventing or treating a Flaviviridae infection. The use of the inventive chimeric polypeptide for producing recombinant viral vectors such as a measles living viral vector is also disclosed.

主权项

1. A method of inducing an immune response against a flavivirus selected from West Nile virus, Japanese encephalitis virus and Yellow fever virus in a subject, comprising:
administering an effective amount of an immunogenic composition comprising a recombinant measles virus vector and a pharmaceutically acceptable vehicle to the subject to thereby induce an immune response in the subject against the flavivirus selected from West Nile virus, Japanese encephalitis virus and Yellow fever virus, wherein the measles virus vector comprises an isolated polynucleotide comprising a coding sequence that encodes a chimeric polypeptide, the chimeric polypeptide comprising at least one subdomain of an E protein bound to at least one subdomain of a membrane M protein, wherein the subdomain of the E protein comprises at least one ectodomain III peptide selected from amino acids 18 to 120 of SEQ ID NO: 20, amino acids 171to 273 of SEQ ID NO: 20, amino acids 324 to 426 of SEQ ID NO: 20, and amino acids 477 to 579 of SEQ ID NO: 20, and wherein the subdomain of the membrane M protein comprises at least one peptide selected from amino acids 123 to 162 of SEQ ID NO: 3, amino acids 154 to 162 of SEQ ID NO: 3, and amino acids 122 to 132 of SEQ ID NO: 12.