Analytical devices having compact lens train arrays

摘要

Apparatus, systems and methods for use in analyzing discrete reactions are provided. The analytical devices of the invention use an array of nanoscale regions (a chip) that has discrete patches of nanoscale regions. The chip mates with a collection device comprising an array of compact lens trains (CLTs) where each of the CLTs corresponds to a single patch of nanoscale regions. Each CLT collects the emitted light from a patch on the chip, collimates the light, performs color separation on the collimated emitted light, and focuses the separated light onto a portion of pixels on the detector below the CLT. Such systems are useful for monitoring many analytical reactions at one time including single molecule sequencing reactions.

主权项

1. A detection system for measuring the emitted light from an array of patches of nanoscale regions comprising:
a chip having an array of patches of nanoscale regions, each patch of nanoscale regions having an array of nanoscale regions; wherein light is emitted from the nanoscale regions from at least a first emitter and a second emitter, each emitting a different spectral range of light; an emitted light collection system comprising an array of compact lens trains (CLT's), each CLT corresponding to a patch of nanoscale regions, wherein each CLT comprises:
a collimating lens for collimating light from the emission sources;a color separating element for spectrally separating light; anda focusing lens for focusing light from the color separating element; and a detector comprising an array of pixels onto which the focusing lens focuses light; whereby images of the nanoscale regions are focused onto the detector such that some pixels on the detector detect signal corresponding to the first spectral range, and some pixels on the detector detect signal corresponding to the second spectral range, thereby separately detecting light corresponding to the first emitter and the second emitter over time.