发明名称 |
Porphyrin—modified antimicrobial peptides for application as indicators of microbial targets |
摘要 |
Porphyrin-modified antimicrobial peptides as described here may be used as indicators of the presence of microbial targets. Their application may be as (for example) (1) fluorescent indicators in a microarray format, (2) fluorescence or absorbance based indicators in traditional solution based applications, or (3) reflectance based indicators for use in reagent-less detection platforms. |
申请公布号 |
US9581594(B2) |
申请公布日期 |
2017.02.28 |
申请号 |
US201414208189 |
申请日期 |
2014.03.13 |
申请人 |
The United States of America, as represented by the Secretary of the Navy |
发明人 |
Taitt Chris Rowe;White Brandy J. |
分类号 |
G01N33/569;G01N33/58;D06M10/00;D06M10/08;D06M13/07;D06M13/352;D06M13/513;D06M16/00 |
主分类号 |
G01N33/569 |
代理机构 |
US Naval Research Laboratory |
代理人 |
US Naval Research Laboratory ;Roberts Roy |
主权项 |
1. A method of detecting bacterial cells, comprising:
providing a porphyrin-modified antimicrobial peptide comprising a porphyrin covalently attached to an antimicrobial peptide; contacting the porphyrin-modified antimicrobial peptide in a microtiter plate with the bacterial cells, thereby causing a conformational change in the porphyrin-modified antimicrobial peptide; and detecting with a microtiter plate reader a change in fluorescence and/or absorbance of the porphyrin-modified antimicrobial peptide caused by the conformational change, corresponding to a presence or absence of the bacterial cells, wherein the method is operable to detect the bacterial cells without use of an additional optically-active reagent, wherein the porphyrin is 5-mono(4-carboxyphenyl)-10,15,20-triphenyl porphine and the antimicrobial peptide is covalently attached to the porphyrin via carbodiimide-mediated coupling, and wherein said antimicrobial peptide is Indolicidin or Cecropin A-melittin hybrid peptide CeMe(L). |
地址 |
Washington DC US |