CRISPR hybrid DNA/RNA polynucleotides and methods of use

摘要

The present disclosure provides DNA-guided CRISPR systems; polynucleotides comprising DNA, RNA and mixtures thereof for use with CRISPR systems; and methods of use involving such polynucleotides and DNA-guided CRISPR systems.

主权项

1. A single polynucleotide comprising:
a targeting region that comprises deoxyribonucleic acid (DNA) or ribonucleic acid (RNA); and an activating region adjacent to said targeting region that comprises DNA; wherein said activating region comprises a stem loop structure and is capable of binding with a Cpf1.