| 发明名称 | Method for endotoxin removal | ||
| 摘要 | The present invention relates to a method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding endotoxin and an outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%. | ||
| 申请公布号 | US9580692(B2) | 申请公布日期 | 2017.02.28 |
| 申请号 | US201314380477 | 申请日期 | 2013.02.27 |
| 申请人 | GE Healthcare BioProcess R&D AB | 发明人 | Adielsson Patrik;Soderman Tobias E.;Akerblom Anna |
| 分类号 | C12N7/02;B01D15/36;C12N7/00;C07K1/20;B01D15/38;B01J20/24;B01J20/285;B01J20/286 | 主分类号 | C12N7/02 |
| 代理机构 | Wood IP LLC | 代理人 | Wood IP LLC |
| 主权项 | 1. A method for endotoxin removal from a sample comprising the following steps: combining the sample comprising one or more target molecule(s) and an endotoxin with a chromatography media comprising beads having an inner porous core functionalized with ligands capable of binding the endotoxin and a 5-7 μm thick outer porous layer without functional groups and a pore size small enough to exclude the target molecule from the inner core, wherein the exclusion limit of said outer porous layer is about 700 kDa; and collecting the sample from the media, wherein the sample comprises an endotoxin level which is at least 75% less, preferably 90% less, than before the removal and the yield of the target molecule is at least 75%. | ||
| 地址 | Uppsala SE | ||