Transgenic mice expressing human pyrin domain only protein 2

摘要

A transgenic mouse expressing the human gene for PYRIN domain-only protein 2 (POP2). POP2, when expressed in the transgenic mouse model, broadly dampens inflammatory cytokine production, in part through restricting the activation of both Nlrp3 and Aim2 inflammasomes. POP2 mice exhibit reduced susceptibility to LPS- and bacteria-induced septic shock. Further, POP2 mice are less susceptible to the fatal, acute inflammatory pneumonia caused by pulmonary infection with F. tularensis LVS and F. novicida, which are highly pathogenic to mice, but non-pathogenic to humans.

主权项

1. A transgenic mouse whose genome comprises a human pyrin-domain only protein 2 (POP2) transgene comprising 2000 base pairs upstream of a human POP2 gene coding region, a single POP2 exon coding sequence, and a 3′ untranslated region sequence, wherein the mouse expresses the POP2 transgene and has a phenotype of reduced production of proinflammatory cytokines in response to LPS-induced shock and bacterial infections when compared to a control mouse.