MUTANT HEMAGGLUTININ COMPLEX PROTEIN, AND METHOD FOR CULTURING PLURIPOTENT STEM CELLS USING SAME

摘要

Provided are a novel hemagglutinin with which it is possible to remove cells rising above the undifferentiated state as occurs in colonies during culture of pluripotent stem cells, and a novel method for culturing pluripotent stem cells using hemagglutinin. The present invention pertains to a mutant hemagglutinin complex protein derived from Clostridium botulinum type B, wherein the complex protein of the mutant hemagglutinin complex protein includes at least subcomponents HA2 and HA3 of hemagglutinin derived from Clostridium botulinum type B, and at least one amino acid constituting a glycosylation site has mutated. The present invention pertains to a method for culturing pluripotent stem cells, wherein the method includes conducting cell culture in the presence of the disclosed mutant hemagglutinin complex protein.