| 摘要 |
Disclosed is a method of detecting an analyte in a sample, the method comprising: i) flowing through a microfluidic device comprising one or more microchannels, a) the sample, b) a sensor molecule comprising a domain that binds the analyte, a chemiluminescent donor domain and an acceptor domain, wherein the separation and relative orientation of the chemiluminescent donor domain and the acceptor domain, in the presence and/or the absence of analyte, is within ± 50% of the Forster distance, and wherein the sensor molecule is not fixed to the device, c) a substrate of the chemiluminescent donor, ii) mixing the sensor molecule, sample and substrate in the device, and iii) detecting modification of the substrate by the chemiluminescent donor using an electro-optical sensing device and calculating as a ratio the energy transfer occurring between the chemiluminescent donor domain and the acceptor domain, wherein the spatial location and/or dipole orientation of the chemiluminescent donor domain relative to the acceptor domain is altered when the analyte binds the sensor molecule. |
