COMPOUNDS AND CONJUGATES FOR IDENTIFYING AND SEPARATING POST-TRANSLATIONALLY MODIFIED ANALYTES

摘要

Disclosed herein are embodiments of compounds, conjugates, and devices, such as columns comprising such compounds and/or conjugates, that can be used to identify, separate, and quantify post-translationally modified analytes. The disclosed compounds and conjugates can be used to discriminate between analytes, such as peptides, having different post-translation modifications, such as methylations, phosphorylations, acetylations, citrullinations, hydroxylations, nitrosylations, ADP-ribosylations, glycosylations, propionylations, butyrylations, crotonylations, 2-hydroxyisobutyrylations, malonylations, succinylations, formylations, ubiquitinations, neddylations, proline cis-trans isomerizations. In particular disclosed embodiments, the compounds and conjugates can be used to separate peptides having different degrees of methylation.

主权项

1. A conjugate, comprising:
a compound having a structure satisfying Formula I wherein each of A, E, G, and J independently is —N(Rb)2; halogen; —SO3−, —CO2−; aryl; heteroaryl; -linker-aryl; or -linker-heteroaryl; each of L, M, N, and Q independently is —ORb; —O−; —SH; —S−; —N(Rb)2, -linker-aryl; or -linker-heteroaryl; each of X1, X2, X3, and X4 independently is CH2, O, S, or NRb; n is an integer selected from 1 to 3; each Rb independently is hydrogen, aliphatic, heteroaliphatic, aryl, or heteroaryl; and a support component coupled to at least one of A, E, G, J, L, M, N, or Q.